Zika virus

Zika virus is a kind of arbovirus. It belongs to Flaviviridae family, genus Flavivirus. Dengue virus, Yellow fever virus and West Nile virus are also members of this family. Zika virus was first isolated from a rhesus monkey in the Zika forest in Uganda in 1947.

Zika virus is a single stranded RNA virus with two major lineages: Asian and African. The key Zika virus vector is thought to be the daytime biting (indoors and outdoors), urban dwelling Aedesaegypti mosquito.

Zika virus genome consists of a single-stranded positive sense RNA molecule with 10794 kb of length with 2 flanking noncoding regions (5’ and 3’ NCR) and a single long open reading frame encoding a polyprotein: 5’-C-prM-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5-3’. The polyprotein is cleaved into the structural proteins C, M and E and the nonstructural proteins which carry out functions in replication and assembly.

cDNA Clones

  • Zika virus(ZIKV)(ZikaSPH2015) Nonstructural protein 1 / Zika-NS1 natural ORF mammalian expression plasmid
  • Zika virus (ZIKV)(ZikaSPH2015) Nonstructural protein 3 / Zika-NS3 natural ORF mammalian expression plasmid
  • Zika virus (ZIKV)(ZikaSPH2015) Nonstructural protein 5 / Zika-NS5 natural ORF mammalian expression plasmid
  • Zika virus (ZIKV)(ZikaSPH2015) Capsid protein / Zika-C natural ORF mammalian expression plasmid
  • Zika virus (ZIKV)(ZikaSPH2015) prM protein and Envelope / Zika-prM and E natural ORF mammalian expression plasmid

Recombinant Proteins

  • Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E / Envelope protein (Domain III, Fc Tag)
  • Zika virus (ZIKV) (strain Zika SPH2016) ZIKV-E (Stem/anchor domain of flavivirus envelope glycoprotein E) protein (Fc Tag)
  • Zika virus (ZIKV) (strain Zika SPH2015) M / Membane protein (Fc Tag)
  • Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 protein (His Tag)

Antibodies

Anti-Zika antibodies ZKA64 and ZKA78 have been published in a recent Science paper by Stettler et al (Specificity, cross-reactivity and function of antibodies elicited by Zika virus infection., Science. 2016 Jul 14. pii: aaf8505., PubMedID: 27417494).

  • Anti-E-protein DIII (ABDE) [ZV-2]
  • Anti-E-protein DIII (C-C’) [ZV-48]
  • Anti-E-protein DIII (C-C’) [ZV-64]
  • Anti-E-protein DIII (LR) [ZV-67]
  • Anti-Envelope dimer Epitope 1 [752-2C8]
  • Anti-Envelope dimer Epitope 1 [753(3) C10]
  • Anti-Envelope dimer Epitope 2 [747(4) A11]
  • Anti-Envelope dimer Epitope 2 [747(4) B7]
  • Anti-Envelope protein [ZKA64]
  • Anti-Envelope protein [ZKA78]
  • Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]
  • Anti-Neutralizing Epitope [ZKA185]

RNA In Situ Hybridization Probes

The researchers from Washington University found that Zika virus can live in eyes and have identified genetic material from the virus in tears. Abundant ZIKV RNA was apparent in bipolar and ganglion cell neurons of neurosensory retina, optic nerve, and cornea of infected mice as shown by in situ hybridization. The research, in mice, helps explain why some Zika patients develop eye disease, including a condition known as uveitis that can lead to permanent vision loss.

Zika Virus Infection in Mice Causes Panuveitis with Shedding of Virus in Tears. Miner et al., 2016, Cell Reports 16, 1–11 September 20, 2016.