Dendritics generated rat monoclonal antibody (mAb) that recognizes mouse plasmacytoid dendritic cells (pDCs). The target molecule was found to be BST2 (bone marrow stromal cell antigen 2). This antibody, named 120G8, stains a small subset of CD11c-low spleen cells with high specificity. This population produces high amounts of IFNα upon in vitro viral stimulation. Both ex vivo- and in vitro-derived 120G8+ cells display a phenotype identical with that of mouse pDCs (B220-high Ly6C-high Gr1-low CD11b- CD11c-low). Mice treated with 120G8 mAb are depleted of B220-high Ly6C-high CD11c-low cells and have a much reduced ability to produce IFNα in response to in vivo CpG stimulation. mAb 120G8 stains all and only B220-high Ly6C-high CD11c-low pDC in all lymphoid organs. Immunohistochemical studies performed with this mAb indicate that pDC are
located in the T cell area of spleen, lymph nodes, and Peyer’s patches. Using 120G8 mAb in immunofluorescence studies demonstrates mouse strain- and organ-specific differences in the frequency of pDCs and other DC subsets (Asselin-Paturel C et al, 2003 ; J. Immunol., 172:6466; Blasius AI, 2006, J. Immunol., 177:3260 ; Goubier A et Al, 2008, Immunity, 29:464-475).
Clone: 120G8
Species: rat
Specificity: mouse pDCs/IFN producing cells (IPC) (extracellular domain)
Immunogen: mouse plasmatocytoid DCs (pDCs)
Isotype: IgG1/kappa
Species cross-reactivity: nd
Applications tested: Flow cytometry, in vivo depletion, IHC
Usage recommendation: This monoclonal antibody may be used between at 1-10 µg/ml. For pDCs in vivo depletion in Balb /c mice, mAb 120G8 was used between 50-200 µg /
injection.
At Absolute Antibody we have developed a collection of recombinant engineered antibodies against clinically relevant proteins, including homologues of current therapeutic targets. We can match the antibody species to the host organism and tailor the effector function, similar to how pharmaceutical companies develop human therapeutics.
Target (mouse)
Clone ID
Original Format
CD134
OX86
Rat IgG1
CD200
OX90
Rat IgG2a
CD28
D665
Mouse IgG1
CD28
E18
Mouse IgG2b
CD47
mIAP301
Rat IgG2a
GITR
YGITR 860.103.5
Rat IgG2b
GITR
YGITR 765
Rat IgG2b
GITRL
YGL 386
Rat IgG1
ICOS
7E.17G9
Rat IgG2b
LAG3
YAML 555.6
Rat IgG2b
PD-1
RMP1-14
Rat IgG2a
PD-L1
alphaPD-L1
Chicken scFv
PD-L1
YDC 127.1.1
Rat IgG2a
Antibodies against proteins involved in co-stimulation and other aspects of immune cell regulation are of particular interest to therapeutics developers. Some have already entered the clinic, with more in the development pipeline. However, many aspects of immune cell signalling are still unknown, and researchers require ever more advanced tools to tap into this potential.
At Absolute Antibody, we use recombinant technology to provide superior monoclonal antibody reagents at competitive prices. In particular we can modify antibody species and isotype for greater flexibility in vivo, for example we can readily generate mouse-anti-mouse or rat-anti-rat antibodies.
Why go recombinant?
Because of their recombinant manufacture our antibodies show minimal batch-to-batch variability and have potential for customisation. We can convert any antibody into any format allowing us to offer each specificity in a range of species, isotypes and subtypes. This means our customers may ‘build’ an antibody to best suit their experiment.
Choose primary antibody format to suit your secondary reagent
Choose antibody species to be compatible with your model organism
Choose antibody isotype to investigate your chosen host responses (includes IgM and all IgG subtypes)
Choose from a range of custom engineering options such as our Fc Silent format with reduced FcR binding to remove Fc receptor function, or other such formats found in the literature (e.g. IgG1-LALA, IgG1-D265A)
Choose one of our listed antibodies, or apply our recombinant technology to your own clone. All our antibody services are royalty-free.
The immune system fights off pathogens, but this defensive force can be pathogenic itself when hyperactive, resulting in autoimmune diseases such as lupus and multiple sclerosis. Consequently, the body has developed multiple mechanisms to suppress the immune system when necessary.
One method of immunosuppression is the PD-1 pathway. This pathway is activated in response to the mobilization of the immune system. The receptor PD-1 is expressed on the surface of activated lymphocytes. Similarly, its ligand, PD-L1, is expressed by antigen-presenting cells in response to cytokine signaling. When PD-L1 is bound to PD-1, downstream signaling undoes the phosphorylation events associated with activation, thereby reverting lymphocytes to an inactive state [1, 2].
IHC of paraffin-embedded human ovary tumor tissue slide using PD-1/CD279 Antibody,at dilution of 1:200 (under 10x lens).
Immunotherapy: A Promising Treatment for Cancer
Tumor cells take advantage of the PD-1 pathway to evade the immune system [3]. Consequently, many pharmaceutical companies have been developing drugs to inhibit PD-1 and PD-L1. In clinical trials, many patients have shown strong responses to these therapies [4-10]. For these drugs to be most effective, a high number of CD8+ T cells must already be at the tumor site, ready to be mobilized after inhibition of the PD-1 pathway [11].Recent investigations have uncovered promising methods of increasing the efficacy of PD1 inhibitors. One method is combining PD1 inhibitors with other drugs, such as HDAC inhibitors [12] and anti-CTLA4 antibodies [9]. Another method is using biomarkers to predict response to therapy [13,14]. Recent work has also suggested that GSK3 inhibitors can enhance effects of immunotherapy [15].Though these results have been encouraging, several challenges remain, including the mitigation of autoimmune effects and how to overcome drug resistance.
1. Ohegbulam et al. (2015) Human cancer immunotherapy with antibodies to the PD-1 and PD-L1 pathway. Trends Mol Med. 21:24-33.
2. Haanen, J. (2013) Immunotherapy of Melanoma. EJC Suppl 11:97-105.
3. Yao et al. (2013) Advances in targeting cell surface signaling molecules for immune modulation. Nat Rev Drug Discov. 12:130-146.
4. Topalian, S. et al. (2012) Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N. Engl. J. Med. 366:2443-2454.
5. Hamid, O. et al. (2013) Safety and tumor responses with lambrolizumab (anti-PD-1) in melanoma. N. Engl. J. Med. 369:134-144.
6. Topalian, S. L. et al. (2012) Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N. Engl. J. Med. 366:2443–2454
7. Brahmer, J. R. et al. (2012) Safety and activity of anti-PD-L1 antibody in patients with advanced cancer. N. Engl. J. Med. 366:2455–2465
8. Hamid, O. et al. (2013) Safety and tumor responses with lambrolizumab (anti-PD-1) in melanoma. N. Engl. J. Med. 369:134–144
9. Wolchok, J. D. et al. (2013) Nivolumab plus ipilimumab in advanced melanoma. N. Engl. J. Med. 369:122–133
10. Topalian, S. L. et al. (2014) Survival, durable tumor remission, and long-term safety in patients with advanced melanoma receiving nivolumab. J. Clin. Oncol. 32:1020–1030
11. Tumeh, P. et al. (2014) PD-1 blockade induces responses by inhibiting adaptive immune response. Nature. 515:568-71.
12. Woods, D. et al. (2015) HDAC inhibition upregulates PD-1 ligands in melanoma and augments immunotherapy with PD-1 blockade. Cancer Immunol Res 12:1375-85.
13. Chakravarti, N., & Prieto, V. G. (2015). Predictive factors of activity of anti-programmed death-1/programmed death ligand-1 drugs: immunohistochemistry analysis. Translational Lung Cancer Research, 4:743–751.
14. Barak, V. et al. (2015) Assessing response to new treatments and prognosis in melanoma patients, by the biomarker S-100B. Anticancer Res. 35:6755-60.
15. Taylor, A. et al. (2014) Glycogen synthase kinase 3 inactivation drives T-bet-mediated downregulation of co-receptor PD-1 to enhance CD8+ cytolytic T cell responses. Immunity 44:274-86.
*Up to HKD300 credit for each order; valid until 28/06/2017
Terms & Conditions
Buy 1 antibody to redeem HKD50 credit, 2 antibodies to redeem HKD150 credit, and 3 antibodies to redeem HKD300 credit.
Valid for R&D Systems antibodies from 100ug / 100ul / 100test or larger sizes.
Valid until 28/6/2017.
Promotion not valid in conjunction with other offers or discounts.
Promotion valid for single order only and cannot be accumulated.
ATCG reserves the right to explain the terms of this promotion.
New antibody lots are screened by application alongside the previous commercial lots. This ensures that antibody specificity and sensitivity remains consistent from lot-to-lot. Each manufactured lot Goat Anti-Human Lipocalin-2 Polyclonal Antibody (Catalog# AF1757; 15 ug/mL) was screened in human pancreatic cancer tissue.
HK$400 for any 40µg Neuroscience Antibodies from GenScript
GenScript offers a wide selection of neuroscience antibodies for Alzheimer’s disease research as well as pathways that may play a role in Parkinson’s disease, and amyotrophic lateral sclerosis.
Unique collection of phospho-specific antibodies and their non-phospho pairs
Full coverage of neurological disease pathways
Multiple applications: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, etc
Terms and conditions:
1. The promotion is valid for HK & Macau customers until 12-May-2017
2. Use promotion code 2017NEURO40 when placing order with ATCG
3. Buy 3 antibodies to waive the shipping fee
