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Flash Sale | BioVision Assay Kits | Summer Promotion


Period: now until Aug 31st, 2017
Product: selected BioVision assay kits listed below
Discount: 50% off
Code: BVATCG1707
Region: Hong Kong & Macau

Name Cat# Size
Lysozyme Activity Assay Kit (Fluorometric) K236 100 assays
Lysozyme Inhibitor Screening Kit K237 100 assays
PicoProbe™ D-2-Hydroxyglutarate Dehydrogenase Assay Kit (Fluorometric) K248 100 assasys
Antibody/Protein Aggregation Assay Kit K308 100 assays
Angiotensin II Converting Enzyme (ACE2) Inhibitor Screening Kit K310 100 assays
Crystal Violet Cell Cytotoxicity Assay Kit K329 1000 assays
Neutral Red Cell Cytotoxicity Assay Kit K447 1000 assays
HDAC6 Inhibitor Screening Kit (Fluorometric) K465 100 assays
HDAC6 Activity Assay Kit (Fluorometric) K466 100 assays
Org Frontier™ Chloroplast Isolation Kit K468 10 g extractions
Neprilysin Activity Assay Kit (Fluorometric) K487 100 assays
Diamine Oxidase Activity Assay Kit (Fluorometric) K496 100 assays
Histamine Assay Kit (Colorimetric) K506 100 assays
Multidrug Efflux Transporter (MDR1/P-gp) Ligand Screening Kit K507 100 Assays
Transglutaminase Inhibitor Screening Assay Kit K508 100 assays
Acidic Mammalian Chitinase Activity Kit (Fluorometric) K513 100 assays
3D Cell Culture Matrix Alginate Hydrogel Kit K517 100 assays
3D Cell Culture Matrix BME Kit K518 100 assays
3D Cell Culture Matrix Duo-Matrix Kit K519 100 assays
Factor XIIIa Activity Assay Kit (Colorimetric) K522 100 assays
Homocysteine Assay Kit (Fluorometric) K531 100 assays
α-L-Fucosidase Activity Assay Kit (Fluorometric) K542 100 assays
Acidic Mammalian Chitinase Inhibitor Screening Kit (Fluorometric) K693 100 assays
Human Hexokinase (HK) Inhibitor Screening Kit (Colorimetric) K713 100 assays
EZClick™ Protein Synthesis Monitoring Assay Kit (Microscopy) K714 50 assays
EZClick™ Protein Synthesis Monitoring Assay Kit (Flow Cytometry) K715 50 assays
Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) K897 100 assays
Methanol Assay Kit (Colorimetric) K898 100 assays
Cell Transformation Assay Kit (Colorimetric) K921 100 assays
Total Phosphodiesterase Activity Assay Kit (Fluorometric) K927 100 assays
3D Cell Culture HTS Cell Viability Complete Assay Kit K948 100 assays
Prostate Specific Antigen (PSA) Activity Assay Kit (Fluorometric) K962 100 assays
Phospho-p38 MAPK (Thr180+Tyr182) Translocation Assay Kit (Cell-Based) K965 100 assays
Cholinesterase Activity Assay Kit (Colorimetric) K975 100 assays
Nitric Oxide Cell-Based HTS Assay Kit K979 100 assays
5-Lipoxygenase Inhibitor Screening Kit (Fluorometric) K980 100 assays
β-Xylosidase Activity Assay Kit (Fluorometric) K981 100 assays
Mutant Isocitrate Dehydrogenase (Mutant IDH) Activity Assay Kit (Colorimetric) K985 100 assays
Methyltransferase Activity Assay Kit (Colorimetric) K986 100 assays
Alkaline Sphingomyelinase Activity Assay Kit (Colorimetric) K987 100 assays
Plasma Kallikrein Inhibitor Screening Kit (Colorimetric) K989 100 assays
3D Cell Culture Ready-to-Use Scaffold Complete Kit K990 100 assays
Factor XIIa Activity Assay Kit (Colorimetric) K994 100 assays
Neprilysin Inhibitor Screening Kit (Fluorometric) K996 100 assays
Plasma Kallikrein Activity Assay Kit (Colorimetric) K997 100 assays


Monoclonal Anti-mouse pDC/IPC/CD317/BST2 (clone 120G8.04)


Dendritics generated rat monoclonal antibody (mAb) that recognizes mouse plasmacytoid dendritic cells (pDCs). The target molecule was found to be BST2 (bone marrow stromal cell antigen 2). This antibody, named 120G8, stains a small subset of CD11c-low spleen cells with high specificity. This population produces high amounts of IFNα upon in vitro viral stimulation. Both ex vivo- and in vitro-derived 120G8+ cells display a phenotype identical with that of mouse pDCs (B220-high Ly6C-high Gr1-low CD11b- CD11c-low). Mice treated with 120G8 mAb are depleted of B220-high Ly6C-high CD11c-low cells and have a much reduced ability to produce IFNα in response to in vivo CpG stimulation. mAb 120G8 stains all and only B220-high Ly6C-high CD11c-low pDC in all lymphoid organs. Immunohistochemical studies performed with this mAb indicate that pDC are
located in the T cell area of spleen, lymph nodes, and Peyer’s patches. Using 120G8 mAb in immunofluorescence studies demonstrates mouse strain- and organ-specific differences in the frequency of pDCs and other DC subsets (Asselin-Paturel C et al, 2003 ; J. Immunol., 172:6466; Blasius AI, 2006, J. Immunol., 177:3260 ; Goubier A et Al, 2008, Immunity, 29:464-475).

Clone: 120G8
Species: rat
Specificity: mouse pDCs/IFN producing cells (IPC) (extracellular domain)
Immunogen: mouse plasmatocytoid DCs (pDCs)
Isotype: IgG1/kappa
Species cross-reactivity: nd
Applications tested: Flow cytometry, in vivo depletion, IHC
Usage recommendation: This monoclonal antibody may be used between at 1-10 µg/ml. For pDCs in vivo depletion in Balb /c mice, mAb 120G8 was used between 50-200 µg /


防伪, 较真就要[一]码归一[码] R&D Systems, Tocris Bioscience, Novus Biologicals


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Recombinant Antibodies for Immunotherapy Research

At Absolute Antibody we have developed a collection of recombinant engineered antibodies against clinically relevant proteins, including homologues of current therapeutic targets. We can match the antibody species to the host organism and tailor the effector function, similar to how pharmaceutical companies develop human therapeutics.

t cell receptor

Target (mouse) Clone ID Original Format
CD134 OX86 Rat IgG1
CD200 OX90 Rat IgG2a
CD28 D665 Mouse IgG1
CD28 E18 Mouse IgG2b
CD47 mIAP301 Rat IgG2a
GITR YGITR 860.103.5 Rat IgG2b
GITR YGITR 765 Rat IgG2b
GITRL YGL 386 Rat IgG1
ICOS 7E.17G9 Rat IgG2b
LAG3 YAML 555.6 Rat IgG2b
PD-1 RMP1-14 Rat IgG2a
PD-L1 alphaPD-L1 Chicken scFv
PD-L1 YDC 127.1.1 Rat IgG2a

Antibodies against proteins involved in co-stimulation and other aspects of immune cell regulation are of particular interest to therapeutics developers. Some have already entered the clinic, with more in the development pipeline. However, many aspects of immune cell signalling are still unknown, and researchers require ever more advanced tools to tap into this potential.
At Absolute Antibody, we use recombinant technology to provide superior monoclonal antibody reagents at competitive prices. In particular we can modify antibody species and isotype for greater flexibility in vivo, for example we can readily generate mouse-anti-mouse or rat-anti-rat antibodies.

Why go recombinant?
Because of their recombinant manufacture our antibodies show minimal batch-to-batch variability and have potential for customisation. We can convert any antibody into any format allowing us to offer each specificity in a range of species, isotypes and subtypes. This means our customers may ‘build’ an antibody to best suit their experiment.

  • Choose primary antibody format to suit your secondary reagent
  • Choose antibody species to be compatible with your model organism
  • Choose antibody isotype to investigate your chosen host responses (includes IgM and all IgG subtypes)
  • Choose from a range of custom engineering options such as our Fc Silent format with reduced FcR binding to remove Fc receptor function, or other such formats found in the literature (e.g. IgG1-LALA, IgG1-D265A)
  • Choose one of our listed antibodies, or apply our recombinant technology to your own clone. All our antibody services are royalty-free.

Soluble B7-H1/PD-L1 Levels in Multiple Cancer Subtypes: High Sensitivity Measurement by Immunoassay


Although the immune checkpoint protein B7-H1/PD-L1 is a commonly used cancer biomarker, controversy remains over the predictive and prognostic utility of immunohistochemical methods. Download our white paper which shows that we can reliably measure B7-H1/PD-L1 in conditioned media supernates and cell lysates from a number of cancer cell lines.

Data from our white paper indicate that:

  • We can reliably measure B7-H1/PD-L1 in a number of human cancer cell lines
  • Soluble B7-H1/PD-L1 expression levels are coincident with loss of PTEN tumor suppressor or increased activation of the phosphoinositide 3-kinase pathway


Request a copy:

The PD-1 / PD-L1 Pathway


The immune system fights off pathogens, but this defensive force can be pathogenic itself when hyperactive, resulting in autoimmune diseases such as lupus and multiple sclerosis. Consequently, the body has developed multiple mechanisms to suppress the immune system when necessary.

One method of immunosuppression is the PD-1 pathway. This pathway is activated in response to the mobilization of the immune system. The receptor PD-1 is expressed on the surface of activated lymphocytes. Similarly, its ligand, PD-L1, is expressed by antigen-presenting cells in response to cytokine signaling. When PD-L1 is bound to PD-1, downstream signaling undoes the phosphorylation events associated with activation, thereby reverting lymphocytes to an inactive state [1, 2].

Antibody Cat no. Type Applications
PD-1/CD279 Antibody 18106-1-AP Rabbit Poly ELISA, WB, FC, IHC
IHC of paraffin-embedded human ovary tumor tissue slide using PD-1/CD279 Antibody,at dilution of 1:200 (under 10x lens).

Immunotherapy: A Promising Treatment for Cancer

Tumor cells take advantage of the PD-1 pathway to evade the immune system [3]. Consequently, many pharmaceutical companies have been developing drugs to inhibit PD-1 and PD-L1. In clinical trials, many patients have shown strong responses to these therapies [4-10]. For these drugs to be most effective, a high number of CD8+ T cells must already be at the tumor site, ready to be mobilized after inhibition of the PD-1 pathway [11].Recent investigations have uncovered promising methods of increasing the efficacy of PD1 inhibitors. One method is combining PD1 inhibitors with other drugs, such as HDAC inhibitors [12] and anti-CTLA4 antibodies [9]. Another method is using biomarkers to predict response to therapy [13,14]. Recent work has also suggested that GSK3 inhibitors can enhance effects of immunotherapy [15].Though these results have been encouraging, several challenges remain, including the mitigation of autoimmune effects and how to overcome drug resistance.

Current Antibody Drug Development

Drug Company
PD-1 SHR-1210 Incyte
Nivolumab Bristol-Myers Squibb
Pembrolizumab Merck
Pidilizumab CureTech
BMS 936559 Bristol-Myers Squibb
PD-L1 Atezolizumab Roche
Durvalumab AstraZeneca
Avelumab Pfizer/Merck
MDX-1105 Bristol-Myers Squibb
CTLA-4 Ipilimumab Bristol-Myers Squibb
Tremelimumab Pfizer/AstraZeneca
Antibody Cat no. Type Applications
PD-L1/CD274 Antibody 17952-1-AP Rabbit Poly ELISA, IF, IHC, IP, WB

KD/KO Validated, 9 Publications

WB result of PD-L1 antibody (17952-1-AP, 1:500) with si-Control and si-PD-L1 transfected HepG2 and HeLa cells with 3 separate constructs.

Related Products

Antibody Cat no. Type Applications
PD-L1/CD274  66248-1-Ig  Mouse mono  ELISA, WB, IHC, IF, FC
CD86/CTLA 4  13395-1-AP  Rabbit poly  ELISA, WB, FC
CD3 epsilon  17617-1-AP  Rabbit poly  ELISA, WB, IHC, IP, FC
GSK3B  22104-1-AP  Rabbit poly  ELISA, WB, IHC, IF, IP
BRAF  20899-1-AP  Rabbit poly  ELISA, IHC, IF


1. Ohegbulam et al. (2015) Human cancer immunotherapy with antibodies to the PD-1 and PD-L1 pathway. Trends Mol Med. 21:24-33.

2. Haanen, J. (2013) Immunotherapy of Melanoma. EJC Suppl 11:97-105.

3. Yao et al. (2013) Advances in targeting cell surface signaling molecules for immune modulation. Nat Rev Drug Discov. 12:130-146.

4. Topalian, S. et al. (2012) Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N. Engl. J. Med. 366:2443-2454.

5. Hamid, O. et al. (2013) Safety and tumor responses with lambrolizumab (anti-PD-1) in melanoma. N. Engl. J. Med. 369:134-144.

6. Topalian, S. L. et al. (2012) Safety, activity, and immune correlates of anti-PD-1 antibody in cancer. N. Engl. J. Med. 366:2443–2454

7. Brahmer, J. R. et al. (2012) Safety and activity of anti-PD-L1 antibody in patients with advanced cancer. N. Engl. J. Med. 366:2455–2465

8. Hamid, O. et al. (2013) Safety and tumor responses with lambrolizumab (anti-PD-1) in melanoma. N. Engl. J. Med. 369:134–144

9. Wolchok, J. D. et al. (2013) Nivolumab plus ipilimumab in advanced melanoma. N. Engl. J. Med. 369:122–133

10. Topalian, S. L. et al. (2014) Survival, durable tumor remission, and long-term safety in patients with advanced melanoma receiving nivolumab. J. Clin. Oncol. 32:1020–1030

11. Tumeh, P. et al. (2014) PD-1 blockade induces responses by inhibiting adaptive immune response. Nature. 515:568-71.

12. Woods, D. et al. (2015) HDAC inhibition upregulates PD-1 ligands in melanoma and augments immunotherapy with PD-1 blockade. Cancer Immunol Res 12:1375-85.

13. Chakravarti, N., & Prieto, V. G. (2015). Predictive factors of activity of anti-programmed death-1/programmed death ligand-1 drugs: immunohistochemistry analysis. Translational Lung Cancer Research, 4:743–751.

14. Barak, V. et al. (2015) Assessing response to new treatments and prognosis in melanoma patients, by the biomarker S-100B. Anticancer Res. 35:6755-60.

15. Taylor, A. et al. (2014) Glycogen synthase kinase 3 inactivation drives T-bet-mediated downregulation of co-receptor PD-1 to enhance CD8+ cytolytic T cell responses. Immunity 44:274-86.

Liver Diseases

Mac-2 binding protein (Mac-2bp), known as 90K, is a highly N-glycosylated, secreted protein, identified as a ligand of Galectin-3. It is considered that through interaction with Galectin-3, Mac-2bp promotes homotypic cell-cell contact or regulates cell adhesion. And it has been reported that Mac-2bp levels in blood have associations with various cancers and chronic hepatic diseases such as NASH (Non-Alcoholic Steatohepatitis).

27362 Human Mac-2 binding protein (Mac-2bp) Assay Kit – IBL

27796 Mouse Mac-2 binding protein (Mac-2bp) Assay Kit – IBL

Hepatocyte growth factor/scatter factor (HGF) is a liver regeneration and growth factor that was isolated and purified from the plasma of the person who suffers fulminant hepatitis. In addition blood and tissue concentrations being assessed during liver injury, increases in concentration in body fluids have been reported in other types of disease besides liver disease (e.g., inflammatory diseases, neoplastic diseases, and fibrosis). HGF is secreted as an inactive single-chain pro-form, and it exerts its physiological activity after processing by HGF activator (HGFA), coagulation factor VIIa, etc., and conversion to activated HGF, a heterodimer. This product is a kit that measures human HGF by the WHO standard.

27402 Human Total HGF Assay Kit

c-Met, the product of c-met gene has been reported to be the HGF receptor since HGF induces phosphorylation of c-Met by binding specifically to it. It is a heterodimer protein composed of an α-chain that is linked to a β-chain. The β-chain has the intracellular tyrosine kinase domain, the transmembrane domain and the extracellular domain, and it is tied into the α-chain that is the extracellular domain of c-Met. c-Met exists mainly in epidermal cells. It is found in the digestive tract, prostate gland, seminal vesicles, mammary gland, microglia cells, monocytes and macrophages, and more amount in the liver and kidney. It is considered that c-Met tansmits signals resulting from binding to HGF, such as growth, motility and organ formation, in the organs and cells mentioned above. This kit is designed to measure Human c-Met.

27407 Human c-Met Assay Kit